Improving Reconstituted HDL Composition for Efficient Post-Ischemic Reduction of Ischemia Reperfusion Injury
| Autor: | Jean-Christophe Prost, Fabrizio Montecucco, Nicolas Vuilleumier, Katia Galan, Richard W. James, François Mach, Miguel Frias, Vincent Braunersreuther, Sandrine Lecour, Aurélien Thomas, Sarah Pedretti, Jonas Brinck, Marie-Claude Brulhart-Meynet, Graziano Pelli |
|---|---|
| Přispěvatelé: | Division of Cardiology, Faculty of Health Sciences |
| Rok vydání: | 2015 |
| Předmět: |
Male
Neutrophils Pharmacology ddc:616.07 chemistry.chemical_compound Mice Ischemia Sphingosine Myocytes Cardiac Hypoxia Phospholipids Cells Cultured Cardioprotection Mitogen-Activated Protein Kinase 1 ddc:616 Multidisciplinary Mitogen-Activated Protein Kinase 3 Reverse cholesterol transport Heart Bioactive compound Reperfusion injury Biochemistry Medicine lipids (amino acids peptides and proteins) medicine.symptom Lipoproteins HDL Research Article STAT3 Transcription Factor Science Inflammation Myocardial Reperfusion Injury Biology medicine Animals Rats Wistar ddc:576 Apolipoprotein A-I Hypoxia (medical) medicine.disease Rats Mice Inbred C57BL chemistry Reperfusion Lysophospholipids Proto-Oncogene Proteins c-akt |
| Zdroj: | PLOS ONE PLOS ONE, Vol. 10, No 3 (2015) P. e0119664 PLoS ONE Plos One, vol. 10, no. 3, pp. e0119664 PLoS One PLoS ONE, Vol 10, Iss 3, p e0119664 (2015) PloS one |
| ISSN: | 1932-6203 |
| Popis: | BackgroundNew evidence shows that high density lipoproteins (HDL) have protective effects beyond their role in reverse cholesterol transport. Reconstituted HDL (rHDL) offer an attractive means of clinically exploiting these novel effects including cardioprotection against ischemia reperfusion injury (IRI). However, basic rHDL composition is limited to apolipoprotein AI (apoAI) and phospholipids; addition of bioactive compound may enhance its beneficial effects.ObjectiveThe aim of this study was to investigate the role of rHDL in post-ischemic model, and to analyze the potential impact of sphingosine-1-phosphate (S1P) in rHDL formulations.Methods and resultsThe impact of HDL on IRI was investigated using complementary in vivo, ex vivo and in vitro IRI models. Acute post-ischemic treatment with native HDL significantly reduced infarct size and cell death in the ex vivo, isolated heart (Langendorff) model and the in vivo model (-48%, pConclusionHDL afford protection against IRI in a clinically relevant model (post-ischemia). rHDL is significantly protective if supplemented with S1P. The protective impact of HDL appears to target directly the cardiomyocyte. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|