Hematopoietic changes induced by a single injection of anti-CD3 monoclonal antibody into normal mice
| Autor: | Asmâa Ben Amor, Michel Dy, Elke Schneider, Véronique Salaün |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
CD3 Complex medicine.drug_class Hamster Spleen Bone Marrow Cells Biology Monoclonal antibody Leukocyte Count Mice medicine Splenocyte Animals Progenitor cell Clonogenic assay Interleukin 3 Granulocyte-Macrophage Colony-Stimulating Factor Cell Biology Molecular biology Hematopoiesis Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure Injections Intravenous Molecular Medicine Female Interleukin-3 Developmental Biology Muromonab-CD3 |
| Zdroj: | Stem cells (Dayton, Ohio). 15(2) |
| ISSN: | 1066-5099 |
| Popis: | The present study evaluates hematopoietic modifications consecutive to in vivo treatment of mice with anti-CD3 monoclonal antibodies (mAb). The hamster mAb 145-2C11, administered in a single i.v. injection of 10 micrograms, induced the release of both interleukin 3 (IL-3) and GM-CSF into the circulation. IL-3 could be detected in the serum within 1 h, attained maximal levels after 4 h and had disappeared after 24 h. Three days later, treated mice exhibited a two- to threefold rise in blood neutrophil levels and increased spleen cell counts. Concomitantly, the incidence of nucleated erythroid cells in these spleens increased around 10-fold, relative to controls having received hamster Ig. At the same time point, clonogenic progenitor frequencies were 10-fold higher in spleens from treated mice than in those from control mice. Furthermore, the responsiveness of these splenocytes to IL-3, in terms of histamine synthesis, was enhanced. In contrast, bone marrow cell populations were only slightly affected by anti-CD3 injection. All hematopoietic changes required multivalent crosslinking of the mAb for induction, since F(ab')2 fragments lacked this activity. A return to normal occurred 7-10 days after treatment. Two i.v. injections of recombinant murine IL-3 together with recombinant murine GM-CSF on a single day had a less pronounced effect on progenitor cell frequencies in the spleen than treatment with anti-CD3. This difference is probably due to the amplification of growth factor-induced hematopoiesis by the interaction with other cytokines generated in response to anti-CD3. |
| Databáze: | OpenAIRE |
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