Supplementary Data from Stromal Cell PD-L1 Inhibits CD8+ T-cell Antitumor Immune Responses and Promotes Colon Cancer

Autor: Aideen E. Ryan, Laurence J. Egan, Thomas Ritter, Philip D. Dunne, Paul Lohan, Niamh A. Leonard, Serika D. Naicker, Kevin Lynch, Oliver Treacy, Grace O'Malley
Rok vydání: 2023
Popis: Characterisation of primary BALB/c MSCs isolated from bone marrow, PD-L1 induction on MSC TNF-TCM is dependent on CT26 NF-κB activity, Validation of scrambled and PD-L1 shRNA transduced Balb/c MSCs - GFP expression and PD-L1 inhibition, Tumour burden following co-administration of either MSCcontrol, MSCTNF-α or MSCTCM, Gating strategy used to analyse CD3, CD4, CD8 and Granzyme B expressing cells in the tumours recovered from animals at Day 21, Dose dependent effects of CT26 co-administered with low and high doses of MSC, Pairwise analysis of relative PD-L1 gene expression between normal and cancer-associated fibroblasts from patients with CRC
Databáze: OpenAIRE