Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum
| Autor: | Sravani Ankati, Vadlamudi Srinivas, Nimmala Naresh, Mahalingam Govindaraj, Rajan Sharma, Sondre Nervik, Nebojsa Simic, Sambangi Pratyusha, Subramaniam Gopalakrishnan, Suraj Prashad Mishra, Susana González |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine genetic structures Metabolite organophosphate education charcoal rot Plant Science Biology Secondary metabolite 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound medicine biocontrol Ecology Evolution Behavior and Systematics Active metabolite Plant stem Ecology Inoculation Botany food and beverages biology.organism_classification Macrophomina phaseolina Plant disease Horticulture 030104 developmental biology chemistry QK1-989 Shoot sorghum sense organs 010606 plant biology & botany medicine.drug |
| Zdroj: | Plants Volume 9 Issue 12 Plants, Vol 9, Iss 1727, p 1727 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9121727 |
| Popis: | Streptomycesalbus strain CAI-21 has been previously reported to have plant growth-promotion abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite were evaluated for their biocontrol potential against charcoal rot disease in sorghum caused by Macrophomina phaseolina. Results exhibited that CAI-21 significantly inhibited the growth of the pathogen, M. phaseolina, in dual-culture (15 mm zone of inhibition), metabolite production (74% inhibition), and blotter paper (90% inhibition) assays. When CAI-21 was tested for its biocontrol potential under greenhouse and field conditions following inoculation of M. phaseolina by toothpick method, it significantly reduced the number of internodes infected (75% and 45% less, respectively) and length of infection (75% and 51% less, respectively) over the positive control (only M. phaseolina inoculated) plants. Under greenhouse conditions, scanning electron microscopic analysis showed that the phloem and xylem tissues of the CAI-21-treated shoot samples were intact compared to those of the diseased stem samples. The culture filtrate of the CAI-21 was purified by various chromatographic techniques, and the active compound was identified as &ldquo organophosphate&rdquo by NMR and MS. The efficacy of organophosphate was found to inhibit the growth of M. phaseolina in the poisoned food technique. This study indicates that S.albus CAI-21 and its active metabolite organophosphate have the potential to control charcoal rot in sorghum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|