Ordered patterning of the sensory system is susceptible to stochastic features of gene expression
| Autor: | Richard W. Carthew, Dimitrios K. Papadopoulos, Ritika Giri, Hemanth K Potluri, Madhav Mani, Pavel Tomancak, Diana M. Posadas |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Transcription
Genetic 0302 clinical medicine Transcription (biology) Gene expression Drosophila Proteins Biology (General) Transvection 0303 health sciences D. melanogaster transvection General Neuroscience 030302 biochemistry & molecular biology Nuclear Proteins General Medicine sensory development Cell biology microRNAs Drosophila melanogaster medicine.anatomical_structure Medicine Female Research Article Sensory Receptor Cells QH301-705.5 Science Sensory system Computational biology Cell fate determination Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences microRNA medicine Animals Allele Gene Psychological repression Alleles 030304 developmental biology Self-organization Stochastic Processes stochasticity General Immunology and Microbiology Sensory neuron Gene Expression Regulation gene expression Protein abundance Developmental biology 030217 neurology & neurosurgery Transcription Factors Developmental Biology |
| Zdroj: | eLife, Vol 9 (2020) Giri, R, Papadopoulos, D K, Posadas, D M, Potluri, H K, Tomancak, P, Mani, M & Carthew, R W 2020, ' Ordered patterning of the sensory system is susceptible to stochastic features of gene expression ', eLIFE, vol. 9 . https://doi.org/10.7554/eLife.53638 eLife |
| DOI: | 10.1101/546911 |
| Popis: | Sensory neuron numbers and positions are precisely organized to accurately map environmental signals in the brain. However, this precision must emerge from biochemical processes within and between cells that are stochastic. We measured intrinsic noise insenselessprotein output, a key determinant of sensory fate, duringDrosophiladevelopment. Perturbing microRNA regulation or genomic locus ofsenselesstranscription produced distinct noise signatures. Genomic location altered protein stochasticity in an allelic-pairing dependent manner (transvection). This generated sensory pattern disorder without perturbing protein abundance. In contrast, loss of microRNA repression ofsenselessincreased protein abundance but not sensory pattern disorder. This suggests that gene expression stochasticity is a critical feature that must be constrained during development to allow rapid yet accurate cell fate resolution.One Sentence SummaryLife on the Margin: balancing speed and accuracy during animal development. |
| Databáze: | OpenAIRE |
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