Protein spectrum changes in exosomes after therapeutic plasma exchange in patients with neuromyelitis optica
| Autor: | Yan-Nan Feng, Yuan Zhuang, Guibin Wang, Tianxin Yang, Lu Yang, Chunya Ma, Wanjun Shen, Zhong Liu, Deqing Wang, Ying Wu, Leiying Zhang, Xiaomin Liu, Yang Yu, Shufang Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Primary Immunodeficiency Diseases Complement factor I 030204 cardiovascular system & hematology Exosomes Exosome Mass Spectrometry 03 medical and health sciences Extracellular matrix protein 1 0302 clinical medicine medicine Humans Lupus Erythematosus Systemic education Autoantibodies Aquaporin 4 Autoimmune disease education.field_of_study Neuromyelitis optica Plasma Exchange business.industry Neuromyelitis Optica Computational Biology Plasmapheresis Hematology General Medicine Middle Aged medicine.disease Complement system Immunoglobulin G Immunology Primary immunodeficiency Female Neuroplastin business Ultracentrifugation 030215 immunology |
| Zdroj: | Journal of Clinical Apheresis. 35:206-216 |
| ISSN: | 1098-1101 0733-2459 |
| DOI: | 10.1002/jca.21781 |
| Popis: | INTRODUCTION Neuromyelitis optica (NMO) is an autoimmune disease with a high rate of blindness and positive for the detection of aquaporin-4 antibody (AQP4) in most patients. NMO acute attacks are managed by high-doses of intravenous methylprednisolone followed by oral taper, and if symptoms fail to resolve, therapeutic plasma exchange (TPE) is added. TPE can remove pathological antibodies and inflammatory factors leading to clinical improvement. METHODS A total of 40 TPE fluid collections from the first to fifth TPE treatments were obtained from eight patients. Exosomes were isolated by ultracentrifugation. Mass spectrometry analyses were used to compare protein change in TPE fluid collection exosomes after the first to the fifth TPE treatments in these patients. RESULTS We detected 647 exosome proteins through data-independent acquisition analysis. It was found that some unknown functional antibody fragments and complement pathway proteins decreased after TPE treatment. The results revealed a significant involvement of the following two key pathways: the primary immunodeficiency and systemic lupus erythematosus that may be associated with NMO pathophysiology and TPE treatment efficacy (P |
| Databáze: | OpenAIRE |
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