Functional identification of the Segregation distorter locus of Drosophila melanogaster by germline transformation
| Autor: | C J Merrill, Barry Ganetzky, Patricia A. Powers, J R McLean |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Genetics biology Restriction Mapping Locus (genetics) Investigations biology.organism_classification Spermatids Germline Restriction fragment Blotting Southern Meiosis Drosophila melanogaster Transformation Genetic Meiotic drive Gene mapping Gene duplication biology.protein Animals Tandem exon duplication Repetitive Sequences Nucleic Acid |
| Zdroj: | Genetics. 137:201-209 |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/137.1.201 |
| Popis: | Segregation Distorter (SD) is a meiotic drive system in D. melanogaster that results in the failure of SD/SD+ males to transmit SD+ homologs owing to the induced dysfunction of spermatids carrying the normal chromosome. Segregation distorter (Sd), the gene primarily responsible for this distorted transmission, is associated with a novel 12-kb restriction fragment containing a tandem duplication of a 5-kb wild-type segment of genomic DNA. When introduced into appropriate genetic backgrounds by germline transformation, this 12-kb fragment causes full levels of distortion and directs the expression of an SD-specific 4-kb transcript. Transformants that have lost part of this segment are unable to cause distortion and do not express the 4-kb transcript. These results identify the tandem duplication as Sd. |
| Databáze: | OpenAIRE |
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