Violacein and its β-cyclodextrin complexes induce apoptosis and differentiation in HL60 cells
| Autor: | Nelson Durán, Mariângela B. M. De Azevedo, Marcela Haun, Giselle Z. Justo, Patricia S. Melo |
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| Rok vydání: | 2003 |
| Předmět: |
Programmed cell death
Indoles Cell Survival HL60 Apoptosis HL-60 Cells DNA Fragmentation Toxicology chemistry.chemical_compound Rosaniline Dyes Humans Coloring Agents Cyclodextrins Caspase 6 biology Nitroblue Tetrazolium beta-Cyclodextrins Caspase 2 Cell Differentiation Biological activity biology.organism_classification Trypanocidal Agents Caspase 9 In vitro chemistry Biochemistry Cell culture Caspases DNA fragmentation Indicators and Reagents Chromobacterium violaceum |
| Zdroj: | Toxicology. 186:217-225 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/s0300-483x(02)00751-5 |
| Popis: | Violacein, a pigment isolated from Chromobacterium violaceum, has been reported to have multiple biological activities including in vitro antitumor effects. Certain anticancer agents are known to induce apoptosis in human tumor cell lines. In this work, our aim was to investigate the effectiveness of violacein/beta-cyclodextrin (beta-CD)-containing systems to produce lethal effects in the human promyelocytic leukemia cell line HL60. Using the MTT tetrazolium reduction test, IC(50) for the inclusion complexes (1:1 and 1:2 violacein:beta-CD molar ratios) and violacein alone were less than 1 microM. Violacein and violacein/beta-CD complexes were able to induce NBT reduction. Moreover, by using the Feulgen reaction, all the compounds were found to trigger apoptosis in HL60 cells, inducing around 35% of DNA fragmentation, as analyzed through the diphenylamine assay. In addition, caspases seem to play an important role in the activation of the executioner phase of apoptosis induced by violacein and its derivatives. |
| Databáze: | OpenAIRE |
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