Effects of acidosis on the structure, composition, and function of adult murine femurs
| Autor: | Alix C. Deymier, Iris Nakashima, Ron Abraham, Mikayla Moody, Tannin A. Schmidt, Anna K. Peterson, David W. Rowe |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
medicine.medical_specialty Bicarbonate Sodium 0206 medical engineering Biomedical Engineering chemistry.chemical_element 02 engineering and technology Biochemistry Bone and Bones Biomaterials chemistry.chemical_compound Mice Form and function Internal medicine medicine Animals Humans Femur Molecular Biology Acidosis Metabolic acidosis General Medicine X-Ray Microtomography 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering Endocrinology chemistry Composition (visual arts) medicine.symptom 0210 nano-technology Function (biology) Biotechnology |
| Zdroj: | Acta biomaterialia. 121 |
| ISSN: | 1878-7568 |
| Popis: | Physiologic pH is maintained in a narrow range through multiple systemic buffering systems. Metabolic Acidosis (MA) is an acid-base disorder clinically characterized by a decrease in systemic pH and bicarbonate (HCO3−) levels. Acidosis affects millions annually, resulting in decreased bone mineral density and bone volume and an increased rate of fracture. We developed an adult murine model of diet-induced metabolic acidosis via graded NH4Cl administration that successfully decreased systemic pH over a 14 day period to elucidate the effects of acidosis on the skeletal system. Blood gas analyses measured an increase in blood calcium and sodium levels indicating a skeletal response to 14 days of acidosis. MA also significantly decreased femur ultimate strength, likely due to modifications in bone morphology as determined from decreased microcomputed tomography values of centroid distance and area moment of inertia. These structural changes may be caused by aberrant remodeling based on histological data evidencing altered OCL activity in acidosis. Additionally, we found that acidosis significantly decreased bone CO3 content in a site-specific manner similar to the bone phenotype observed in human MA. We determined that MA decreased bone strength thus increasing fracture risk, which is likely caused by alterations in bone shape and compounded by changes in bone composition. Additionally, we suggest the temporal regulation of cell-mediated remodeling in MA is more complex than current literature suggests. We conclude that our model reliably induces MA and has deleterious effects on skeletal form and function, presenting similarly to the MA bone phenotype in humans. |
| Databáze: | OpenAIRE |
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