High-resolution crystal structure and in vivo function of a kinesin-2 homologue in Giardia intestinalis
| Autor: | Scott C. Dawson, Jan Löwe, J. C. Hoeng, Joel Mancuso, Jonathan K Pham, Meredith Sagolla, Susan A. House, W Z Cande |
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| Rok vydání: | 2008 |
| Předmět: |
Models
Molecular Cytoplasm Protein Conformation Green Fluorescent Proteins Kinesins Biology Flagellum medicine.disease_cause Crystallography X-Ray Motor protein Cell membrane Evolution Molecular Protein structure Membrane Microdomains Heterotrimeric G protein medicine Giardia lamblia Animals Molecular Biology Cell Membrane Cell Biology Articles Cell biology Protein Structure Tertiary medicine.anatomical_structure Flagella Mutation Kinesin |
| Zdroj: | Molecular biology of the cell. 19(7) |
| ISSN: | 1939-4586 |
| Popis: | A critical component of flagellar assembly, the kinesin-2 heterotrimeric complex powers the anterograde movement of proteinaceous rafts along the outer doublet of axonemes in intraflagellar transport (IFT). We present the first high-resolution structures of a kinesin-2 motor domain and an ATP hydrolysis–deficient motor domain mutant from the parasitic protist Giardia intestinalis. The high-resolution crystal structures of G. intestinalis wild-type kinesin-2 (GiKIN2a) motor domain, with its docked neck linker and the hydrolysis-deficient mutant GiKIN2aT104N were solved in a complex with ADP and Mg2+at 1.6 and 1.8 Å resolutions, respectively. These high-resolution structures provide unique insight into the nucleotide coordination within the active site. G. intestinalis has eight flagella, and we demonstrate that both kinesin-2 homologues and IFT proteins localize to both cytoplasmic and membrane-bound regions of axonemes, with foci at cell body exit points and the distal flagellar tips. We demonstrate that the T104N mutation causes GiKIN2a to act as a rigor mutant in vitro. Overexpression of GiKIN2aT104N results in significant inhibition of flagellar assembly in the caudal, ventral, and posterolateral flagellar pairs. Thus we confirm the conserved evolutionary structure and functional role of kinesin-2 as the anterograde IFT motor in G. intestinalis. |
| Databáze: | OpenAIRE |
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