Transforming growth factor-beta 1 derived from biliary epithelial cells may attenuate alloantigen-specific immune responses
| Autor: | Takashi Maeda, Makoto Hiroi, Saburo Onishi, Yoshihiro Hayashi, Nobuaki Kobayashi, Toshiji Saibara, Katsuhiro Narumoto, Hideaki Enzan, Eriko Miyazaki |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Isoantigens chemical and pharmacologic phenomena Lymphocyte Activation Immune system Transforming Growth Factor beta Rats Inbred BN Cytotoxic T cell Animals Transplantation Homologous Blood Transfusion Lymphocytes Transplantation biology Gallbladder hemic and immune systems Epithelial Cells Mixed lymphocyte reaction Acquired immune system Immunohistochemistry Liver Transplantation Rats Rats Inbred ACI Liver Biliary tract Rats Inbred Lew Immunology biology.protein Cancer research Antibody Lymphocyte Culture Test Mixed Spleen Transforming growth factor |
| Zdroj: | Transplant international : official journal of the European Society for Organ Transplantation. 13(1) |
| ISSN: | 0934-0874 |
| Popis: | Immune response to liver allografts may be different from that to other organ transplants since immunological manipulation easily attenuates immune-response to liver allografts. Numerous studies on the alloantigen-specific immune response have been carried out, however, the precise mechanisms involved in this attenuation are not clear yet. Here we suggest the attenuation of alloantigen-specific immune response by TGF-beta 1 derived from biliary epithelial cells. The transforming growth factor-beta 1 (TGF-beta 1) expression in rat liver was examined immunohistologically. Rat biliary epithelial cells (BEC) were purified from the perfused liver and added to allogeneic mixed lymphocyte reaction (allo-MLR) to assess their attenuating potential on allo-MLR and alloantigen-specific cytotoxic T lymphocyte (allo-CTL) induction. Immunohistological investigation revealed the expression of TGF-beta 1 in biliary epithelial cells. Both purified biliary epithelial cells and TGF-beta 1 attenuated allo-MLR and allo-CTL induction in a dose-dependent manner, and anti-TGF-beta 1 antibody partially relieved this attenuation. This study reveals that biliary epithelial cells, the major target cells of allo-antigen specific immune response, contain TGF-beta 1 and that they have a capacity to attenuate allo-MLR and allo-CTL induction. |
| Databáze: | OpenAIRE |
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