A Rapid Method Combining Golgi and Nissl Staining to Study Neuronal Morphology and Cytoarchitecture
| Autor: | Nadia Pilati, Revers Donga, Martine Hamann, Sofoklis Panteleimonitis, Matthew C. Barker |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Dorsal cochlear nucleus
Pathology medicine.medical_specialty Silver Histology Context (language use) Biology Article Cresyl violet chemistry.chemical_compound symbols.namesake Oxazines medicine Animals Coloring Agents Neurons Staining and Labeling Brain Golgi apparatus Isoquinolines Benzoxazines Rats Staining medicine.anatomical_structure nervous system chemistry Cytoarchitecture symbols Biophysics Nissl body Neuron Anatomy |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 56:539-550 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1369/jhc.2008.950246 |
| Popis: | The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron's histological location by subsequent Nissl staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet–labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet staining is inadequate to quantify differences in soma sizes. (J Histochem Cytochem 56:539–550, 2008) |
| Databáze: | OpenAIRE |
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