LARP6C orchestrates post-transcriptional reprogramming of gene expression during hydration to promote pollen tube guidance

Autor: Celso G. Litholdo, David Honys, Vinod Kumar, Said Hafidh, Marie-Christine Carpentier, Katarina Kulichova, Elodie Billey, Claire Picart, Eric Maréchal, Viviane Jean, Maria R. Conte, Cécile Bousquet-Antonelli, Isabel Cruz-Gallardo, Jean-Marc Deragon
Přispěvatelé: Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Pollen Biology, Institute of Experimental Botany of the Czech Academy of Sciences (IEB / CAS), Czech Academy of Sciences [Prague] (CAS)-Czech Academy of Sciences [Prague] (CAS), Randall Centre for Cell and Molecular Biophysics, King's College London, London SE1 1UL, United Kingdom., LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Department of Chemistry, King‘s College London, GACR grant numbers 17-23203S and 18-02448S- European Regional Development Fund-Project 'Centre for Experimental Plant Biology' (No. CZ.02.1.01/0.0/0.0/16_019/0000738)- Royal Society Newton International fellowship (ref. NF140482)- IUF- BQR - UPVD- PICS du CNRS (LARP&STRESS, n°6170), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-17-CE20-0007,HEAT-EpiRNA,Lecture de l'épitranscriptome du stress thermique chez Arabidopsis(2017), UMR 1200 Laboratoire de Physiologie Cellulaire Végétale, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie Atomique (CEA)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: The Plant cell
The Plant cell, American Society of Plant Biologists (ASPB), 2021, 33 (8), pp.2637-2661. ⟨10.1093/plcell/koab131⟩
Plant Cell
The Plant cell, American Society of Plant Biologists (ASPB), 2021, ⟨10.1093/plcell/koab131⟩
The Plant cell, 2021, 33 (8), pp.2637-2661. ⟨10.1093/plcell/koab131⟩
ISSN: 1040-4651
1532-298X
Popis: Increasing evidence suggests that posttranscriptional regulation is a key player in the transition between mature pollen and the progamic phase (from pollination to fertilization). Nonetheless, the actors in this messenger RNA (mRNA)-based gene expression reprogramming are poorly understood. We demonstrate that the evolutionarily conserved RNA-binding protein LARP6C is necessary for the transition from dry pollen to pollen tubes and the guided growth of pollen tubes towards the ovule in Arabidopsis thaliana. In dry pollen, LARP6C binds to transcripts encoding proteins that function in lipid synthesis and homeostasis, vesicular trafficking, and polarized cell growth. LARP6C also forms cytoplasmic granules that contain the poly(A) binding protein and possibly represent storage sites for translationally silent mRNAs. In pollen tubes, the loss of LARP6C negatively affects the quantities and distribution of storage lipids, as well as vesicular trafficking. In Nicotiana benthamiana leaf cells and in planta, analysis of reporter mRNAs designed from the LARP6C target MGD2 provided evidence that LARP6C can shift from a repressor to an activator of translation when the pollen grain enters the progamic phase. We propose that LARP6C orchestrates the timely posttranscriptional regulation of a subset of mRNAs in pollen during the transition from the quiescent to active state and along the progamic phase to promote male fertilization in plants.
Databáze: OpenAIRE
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