Medium-induced fragility ofSchwanniomyces

Autor:	Teena Walker, Ivan Curran, Byron F. Johnson
Rok vydání:	1994
Předmět:	chemistry.chemical_classification Lysis Starch General Medicine Biology Phosphate Microbiology Yeast Culture Media Phosphates Chemically defined medium chemistry.chemical_compound Enzyme chemistry Biosynthesis Biochemistry Saccharomycetales Secretion Molecular Biology
Zdroj:	Antonie van Leeuwenhoek. 65:107-109
ISSN:	1572-9699 0003-6072
DOI:	10.1007/bf00871752
Popis:	Schwanniomyces occidentalis has attracted interest because of its ability to metabolize starch and similar complex carbohydrates. Studies have been undertaken, mostly using defined media, to ascertain conditions for optimal production and secretion of hydrolytic enzymes. Here we demonstrate the fragility of Schw. occidentalis in many defined media. We especially examined viability in YNB (Yeast Nitrogen Base) plus 1% glucose. Without phosphate supplementation, viability was routinely very low at stationary phase (usually less than 37%), whereas viability of stationary-phase cultures in phosphate-supplemented YNB usually exceeded 97%. The negative implications of having many, presumably permeabilized, dead cells present in assays for secretion of enzymes by living yeast cells are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::868572726a6ddc3f00f3d0551fa0b287 https://doi.org/10.1007/bf00871752 Zobrazit plný text záznamu Full text from SpringerLink