Fluoride-stimulated [3H] thymidine uptake in a human osteoblastic osteosarcoma cell line is dependent on transforming growth factor β
| Autor: | Charles Y.C. Pak, Joseph E. Zerwekh, Peter P. Antich, Berenice Y. Reed |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Cell Count Biology Antibodies chemistry.chemical_compound Transforming Growth Factor beta Internal medicine Sodium fluoride Tumor Cells Cultured medicine Humans Orthopedics and Sports Medicine RNA Messenger Bone growth Osteosarcoma Osteoblasts Dose-Response Relationship Drug Cell growth Growth factor Osteoblast DNA Transforming growth factor beta Blotting Northern Molecular biology Endocrinology medicine.anatomical_structure chemistry biology.protein Sodium Fluoride Spectrophotometry Ultraviolet Fluoride Cell Division Thymidine Transforming growth factor |
| Zdroj: | Journal of Bone and Mineral Research. 8:19-25 |
| ISSN: | 0884-0431 |
| DOI: | 10.1002/jbmr.5650080104 |
| Popis: | Controversy exists regarding the effect of fluoride on human osteoblast proliferation. To learn more of the cellular action of fluoride, we chose the clonal osteoblast cell line HOS TE85 as a model system. In these phenotypically osteoblast-like cells, sodium fluoride stimulated [3H]thymidine incorporation in a dose-dependent manner over the concentration range 1 x 10(-5)-2 x 10(-4) M. The fluoride-induced stimulation of [3H]thymidine uptake was dependent on cell density, being optimal at subconfluent cell numbers. Stimulation of [3H]thymidine uptake was inhibited by anti-transforming growth factor beta but not by antibody to insulin-like growth factor I or beta 2-microglobulin. Transforming growth factor beta was shown to be a biphasic stimulator of [3H]thymidine uptake in HOS TE85, with maximal stimulation occurring at 0.5 nM transforming growth factor beta. In the presence of fluoride the cells were more sensitive to stimulation by this growth factor, with maximum effect occurring at 0.1 nM. Fluoride did not increase mRNA for transforming growth factor beta following either 8 or 24 h of exposure. We conclude that fluoride activates osteoblast proliferation by modulating the cellular sensitivity to transforming growth factor beta, a known stimulator of bone growth. |
| Databáze: | OpenAIRE |
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