Regulation of hexokinase II and glycogen synthase mRNA, protein, and activity in human muscle
| Autor: | Agostino Consoli, Lawrence J. Mandarino, D. K. Granner, K. A. Cusi, C. Sewell, Haruhiko Osawa, R. M. O'Doherty, R. L. Printz, P. Kinchington, Ralph A. DeFronzo |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Blood Glucose Male medicine.medical_specialty Monosaccharide Transport Proteins Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Muscle Proteins Biology Carbohydrate metabolism chemistry.chemical_compound Hexokinase Physiology (medical) Internal medicine Gene expression medicine Hyperinsulinemia Humans Insulin RNA Messenger Glycogen synthase Glucose Transporter Type 4 Muscles Skeletal muscle Glucose clamp technique medicine.disease Glycogen Synthase Endocrinology medicine.anatomical_structure chemistry Glucose Clamp Technique biology.protein Female |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 269:E701-E708 |
| ISSN: | 1522-1555 0193-1849 |
| Popis: | Insulin regulates the activity of key enzymes of glucose metabolism in skeletal muscle by altering transcription or translation or by producing activity-altering modifications of preexisting enzyme molecules. Because of the small size of percutaneous muscle biopsies, these phenomena have been difficult to study in humans. This study was performed to determine how physiological hyperinsulinemia regulates the activities of hexokinase (HK), glycogen synthase (GS), and GLUT-4 in human skeletal muscle in vivo. We determined mRNA abundance, protein content, and activities for these proteins in muscle biopsies before and after a hyperinsulinemic clamp in normal subjects. HK I, HK II, GS, and GLUT-4 were expressed in muscle. HK II accounted for 80% of total HK activity and was increased by insulin from a basal value of 2.11 +/- 0.26 to 3.35 +/- 0.47 pmol.min-1.mg protein-1 (P < 0.05); HK I activity was unaffected. Insulin increased GS activity from 3.85 +/- 0.82 to 6.06 +/- 0.49 nmol.min-1.mg-1 (P < 0.01). HK II mRNA was increased 3.3 +/- 1.3-fold (P < 0.05) by insulin infusion. HK I, GS, and GLUT-4 mRNA and protein were unaffected. Because insulin infusion increased HK II but not GS mRNA, we conclude that HK II and GS may be regulated by insulin by different mechanisms in human skeletal muscle. |
| Databáze: | OpenAIRE |
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