Modelling Asthma in Mice Using Common Aeroallergens

Autor:	Bidisha Paul Chowdhury, Magdalena M. Gorska
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Disease Models Animal Mice Mice Inbred BALB C Ovalbumin Animals Allergens Bronchoalveolar Lavage Fluid Lung Article Asthma
Zdroj:	Methods Mol Biol Methods in Molecular Biology ISBN: 9781071623633
Popis:	Aeroallergens are common inducers of asthma in humans and are widely used in experimental research to generate animal models of this disease. In this chapter, we describe four mouse models of aeroallergen-induced asthma. These models differ in type and number of allergens used, route and duration of allergen exposure, and utilization of an adjuvant, representing different mechanistic variants of asthma. In addition, we describe several basic methods that are commonly used in mechanistic studies of asthma in mice. These methods include tracheotomy and bronchoalveolar lavage, cytospin and morphologic analysis of bronchoalveolar lavage cells, and lung harvest and digestion for generation of single-cell suspension.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8680db2cad1fe17155d81ed4e4f42c26 https://europepmc.org/articles/PMC9721467/ Zobrazit plný text záznamu