Analysis of chronic lung transplant rejection by MALDI-TOF profiles of bronchoalveolar lavage fluid
| Autor: | Christine H. Wendt, Gary L. Nelsestuen, Tereza Cervenka, Matthew S. Wroblewski, Yan Zhang, Marshall I. Hertz |
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| Rok vydání: | 2006 |
| Předmět: |
Graft Rejection
medicine.medical_specialty Bronchiolitis obliterans Disease Biochemistry Gastroenterology Sensitivity and Specificity chemistry.chemical_compound Postoperative Complications Lung transplant rejection Internal medicine medicine Disease biomarker Humans Uteroglobin Molecular Biology Bronchiolitis Obliterans Retrospective Studies Lung medicine.diagnostic_test business.industry Syndrome medicine.disease medicine.anatomical_structure Bronchoalveolar lavage chemistry Lung disease Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Immunology Chronic Disease Feasibility Studies Muramidase Lysozyme business Bronchoalveolar Lavage Fluid Biomarkers Lung Transplantation |
| Zdroj: | Proteomics. 6(3) |
| ISSN: | 1615-9853 |
| Popis: | While lung transplant is an effective therapy for advanced lung disease, chronic allograph rejection remains a primary basis for lower survival rates than those for other solid organ transplants. This study used carefully controlled Zip-Tip extraction of bronchoalveolar lavage fluid (BALF) followed by MALDI-TOF MS to identify biomarkers of chronic lung transplant rejection. Many differences were observed between controls, those who did not develop chronic rejection within 100 months, and patients who had developed chronic rejection, diagnosed as bronchiolitis obliterans syndrome (BOS). Intensity ratios of peaks within the same MALDI-TOF profile were used to quantify the result. One of the best identifiers of BOS was a lowered ratio of clara cell protein (CCP m/z = 15 835) to lysozyme (m/z = 14 700), which gave 94% specificity and 74% sensitivity for diagnosis. Furthermore, low values for CCP/Lysozyme ( |
| Databáze: | OpenAIRE |
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