Swine acute diarrhea syndrome coronavirus (SADS-CoV) antagonizes interferon-β production via blocking IPS-1 and RIG-I

Autor:	Zhihai Zhou, Jingyun Ma, Tian Lan, Yao-Rong Tan, Li Qianniu, Xiaoling Yan, Yuan Sun, Xiaoyu Tang
Rok vydání:	2020
Předmět:	Cancer Research Swine viruses RIG-I retinoic acid-inducible gene I medicine.disease_cause PEDV porcine epidemic diarrhea virus RIG-I PRRs pattern recognition receptors TANK-binding kinase 1 IRF-3 IFN regulatory factor 3 Phosphorylation Promoter Regions Genetic MOI multiplicity of infection IPS-1 Coronavirus 0303 health sciences biology Alphacoronavirus NF-kappa B virus diseases MERS-CoV Middle East respiratory syndrome coronavirus SADS-CoV swine acute diarrhea syndrome coronavirus respiratory system Interferon beta mRNA messenger RNA Sendai virus IFN-β interferon beta Infectious Diseases CPE cytopathic effect Host-Pathogen Interactions DEAD Box Protein 58 Signal transduction Coronavirus Infections TLR3 toll-like receptor 3 IPEC-J2 porcine small intestinal epithelial cells Signal Transduction MDA5 melanoma differentiation-associated gene 5 TGEV transmissible gastroenteritis virus SARS-CoV severe acute respiratory syndrome coronavirus Active Transport Cell Nucleus behavioral disciplines and activities Article Cell Line 03 medical and health sciences NF-κB nuclear factor-kappaB RT-PCR reverse transcription polymerase chain reaction IKKε inhibitor of κB kinase-ε PBS phosphate buffered saline PAMPs pathogen-associated molecular patterns Virology mental disorders medicine Animals poly(I:C) polyinosinic-polycytidylic acid 030304 developmental biology Cell Nucleus IFA indirect immunofluorescence assay qRT-PCR quantitative real time RT-PCR Innate immune system p.i. post-infection 030306 microbiology Interferon-beta biochemical phenomena metabolism and nutrition NFKB1 biology.organism_classification Immunity Innate SeV Sendai virus TBK1 TANK-binding kinase 1 IPS-1 mitochondria antiviral-signaling protein dsRNA double-stranded RNA Interferon Regulatory Factor-3 IRF3 Swine acute diarrhea syndrome coronavirus
Zdroj:	Virus Research
ISSN:	0168-1702
DOI:	10.1016/j.virusres.2019.197843
Popis:	Highlights • SADS-CoV failed to induce IFN-β expression and inhibited poly (I:C)-or SEV-mediated IFN-β production in IPEC-J2 cells. • SADS-CoV interrupted poly (I:C)-induced phosphorylation and nuclear translocation of IRF3 and NF-κB. • SADS-CoV failed to block IRF3, TBK1 and IKKε activity. • SADS-CoV impeded IFN-β induction mediated by IPS-1 and RIG-I. Swine acute diarrhea syndrome coronavirus (SADS-CoV), a newly emerging enteric coronavirus, is considered to be associated with swine acute diarrhea syndrome (SADS) which has caused significantly economic losses to the porcine industry. Interactions between SADS-CoV and the host innate immune response is unclear yet. In this study, we used IPEC-J2 cells as a model to explore potential evasion strategies employed by SADS-CoV. Our results showed that SADS-CoV infection failed to induce IFN-β production, and inhibited poly (I:C) and Sendai virus (SeV)-triggered IFN-β expression. SADS-CoV also blocked poly (I:C)-induced phosphorylation and nuclear translocation of IRF-3 and NF-κB. Furthermore, SADS-CoV did not interfere with the activity of IFN-β promoter stimulated by IRF3, TBK1 and IKKε, but counteracted its activation induced by IPS-1 and RIG-I. Collectively, this study is the first investigation that shows interactions between SADS-CoV and the host innate immunity, which provides information of the molecular mechanisms underlying SASD-CoV infection.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86806a2d8f0a277bd992f173c0fe45f7 https://doi.org/10.1016/j.virusres.2019.197843 Zobrazit plný text záznamu Full Text from ScienceDirect