Phenotypic Characterization of Leukocytes in Prenatal Human Dermis
| Autor: | Christopher Schuster, Michael Mildner, Christian Fiala, Adelheid Elbe-Bürger, Marion Prior, Christine Vaculik, Georg Stingl, Wolfgang Eppel |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Adolescent CD3 CD11c Human skin Dermatology Biology T-Lymphocytes Regulatory Biochemistry Cell Line Young Adult 03 medical and health sciences Fetus 0302 clinical medicine Immune system Dermis Pregnancy Leukocytes medicine Humans Mast Cells Molecular Biology 030304 developmental biology 0303 health sciences integumentary system Macrophages FOXP3 Forkhead Transcription Factors Cell Biology Middle Aged Molecular biology Pregnancy Trimester First Phenotype medicine.anatomical_structure Cell culture Immune System Langerhans Cells Immunology biology.protein Original Article Female Biomarkers 030215 immunology |
| Zdroj: | The Journal of Investigative Dermatology |
| ISSN: | 0022-202X |
| DOI: | 10.1038/jid.2012.187 |
| Popis: | The adult human skin harbors a variety of leukocytes providing immune surveillance and host defense, but knowledge about their ontogeny is scarce. In this study we investigated the number and phenotype of leukocytes in prenatal human skin (dermal dendritic cells (DDCs), macrophages, T cells (including FoxP3(+) regulatory T cells), and mast cells) to unravel their derivation and to get a clue as to their putative function in utero. By flow cytometry and immunofluorescence, we found a distinction between CD206(+)CD1c(+)CD11c(+) DDCs and CD206(+)CD209(+)CD1c(-) skin macrophages by 9 weeks estimated gestational age (EGA). T cells appear at the end of the first trimester, expressing CD3 intracytoplasmatically. During midgestation, CD3(+)FoxP3(-) and CD3(+)FoxP3(+) cells can exclusively be found in the dermis. Similarly, other leukocytes such as CD117(+) (c-kit) mast cells were not identified before 12-14 weeks EGA and only slowly acquire a mature phenotype during gestation. Our data show at which time point during gestation antigen-presenting cells, T cells, and mast cells populate the human dermis and provide a step forward to a better understanding of the development of the human skin immune system. |
| Databáze: | OpenAIRE |
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