Genetically-Tunable Mechanical Properties of Bacterial Functional Amyloid Nanofibers
| Autor: | Urartu Ozgur Safak Seker, Ebuzer Kalyoncu, Tugce Onur, M. Tarek Abdelwahab, Mehmet Z. Baykara |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Protein nanofibers
Amyloid Mutant Nanofibers Escherichia coli (E. coli) Mechanical properties Nanotechnology Amyloidogenic Proteins 02 engineering and technology 010402 general chemistry medicine.disease_cause Microscopy Atomic Force 01 natural sciences Atomic force microscopy Elastic Modulus Microscopy Electrochemistry medicine Escherichia coli Bio-nano interfaces General Materials Science Protein composition Bacterial biofilm Biochemical properties Elastic modulus Spectroscopy Glycoproteins Protein components Chemistry Escherichia coli Proteins Biofilm Force spectroscopy Proteins Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Nanofiber Biofilms Mutation Biophysics 0210 nano-technology |
| Zdroj: | Langmuir |
| ISSN: | 1520-5827 |
| Popis: | Bacterial biofilms are highly ordered, complex, dynamic material systems including cells, carbohydrates, and proteins. They are known to be resistant against chemical, physical, and biological disturbances. These superior properties make them promising candidates for next generation biomaterials. Here we investigated the morphological and mechanical properties (in terms of Young’s modulus) of genetically-engineered bacterial amyloid nanofibers of Escherichia coli (E. coli) by imaging and force spectroscopy conducted via atomic force microscopy (AFM). In particular, we tuned the expression and biochemical properties of the major and minor biofilm proteins of E. coli (CsgA and CsgB, respectively). Using appropriate mutants, amyloid nanofibers constituting biofilm backbones are formed with different combinations of CsgA and CsgB, as well as the optional addition of tagging sequences. AFM imaging and force spectroscopy are used to probe the morphology and measure the Young’s moduli of biofilm protein nanofibers as a function of protein composition. The obtained results reveal that genetically-controlled secretion of biofilm protein components may lead to the rational tuning of Young’s moduli of biofilms as promising candidates at the bionano interface. |
| Databáze: | OpenAIRE |
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