Design and study of anticaries effect of different medicinal plants against S.mutans glucosyltransferase
| Autor: | Uma Rajeswari Batchu, Shravya Kakulavaram, Shulamithi Repally, Srinivas Bedarakota, Kiranmai Mandava, Namratha Sunkara, Ishwarya Chennuri |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Mouthwashes
Dental Caries Streptococcus mutans 03 medical and health sciences 0302 clinical medicine Bacterial Proteins stomatognathic system Glucosyltransferase medicine Humans Anticaries agent Enzyme Inhibitors Medicinal plants Plants Medicinal biology Traditional medicine Azadirachta indica Plant Extracts Chemistry Chlorhexidine S.mutans Polyherbal mouth wash General Medicine lcsh:Other systems of medicine Azadirachta biology.organism_classification lcsh:RZ201-999 Anti-Bacterial Agents 030205 complementary & alternative medicine Terminalia chebula Kinetics stomatognathic diseases Complementary and alternative medicine Glucosyltransferases Syzygium Drug Design 030220 oncology & carcinogenesis biology.protein Uncompetitive inhibitor Research Article medicine.drug |
| Zdroj: | BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-8 (2019) BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| Popis: | Background The present study was aimed to evaluate the molecular level anticaries effect of different medicinal plants against Streptococcus mutans (S.mutans) glucosyltransferases (gtf). Methods A total of six natural sources named as Terminalia chebula (T.chebula), Psidium guajava (P.guajava), Azadirachta indica (A.indica) and Pongamia pinnata (P.pinnata); two essential oils, clove (Syzygium aromaticum) and peppermint oil (Mentha piperita) were selected as test samples. Hydroalcoholic plant extracts and essential oils were examined for their inhibitory potential on gtf isolated from S.mutans. Polyherbal mouth wash was prepared and its effect on gtf activity was compared with commercial chlorhexidine mouth wash (5%w/v). Enzyme kinetic study was carried out in order to explore the molecular mechanism of enzyme action. Results Out of six natural sources tested, A.indica has shown maximum inhibitory effect of 91.647% on gtf and T.chebula has shown IC50 of 1.091 mg/ml which is significant when compared to standard chlorhexidine. From the final result of kinetic analysis it was found that T.chebula, P.guajava and P.pinnata have show uncompetitive inhibition where as A.indica has shown non-competitive inhibition. Surprisingly, both essential oils have shown allosteric inhibition (sigmoidal response). The polyherbal moutwash has shown significant inhibitory potential on gtf (95.936%) when compared to commercial chlorhexidine mouthwash (p |
| Databáze: | OpenAIRE |
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