Late steps in the assembly of 30 S ribosomal proteins in vivo in a spectinomycin-resistant mutant of Escherichia coli
| Autor: | Hiroko Nashimoto, Hisao Uchida |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Ribosomal Proteins
Spectinomycin Genetic Linkage Protein subunit Mutant Drug Resistance Microbial Biology Ribosomal RNA medicine.disease_cause Diploidy Molecular biology Ribosome assembly Cold Temperature Species Specificity Structural Biology Ribosomal protein Mutation Escherichia coli medicine Ribosomes Molecular Biology Gene medicine.drug |
| Zdroj: | Journal of Molecular Biology. 96:443-453 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(75)90171-0 |
| Popis: | The late steps in ribosome assembly in vivo were studied by characterizing mutations which suppress the cold-sensitivity of a spectinomycin-resistant mutant of Escherichia coli . The results obtained indicated that the cold-sensitivity could be relieved by secondary alterations in either the S2, S3 or S5 protein of the 30 S ribosomal subunit. The gene controlling the alteration of S2 protein was closely linked to the polC gene located at about 3.5 minutes on the genetic map of E. coli , whereas S3 and S5 suppressor genes were linked to the str-spc region at 64 minutes. A possible model in which the S2, S3 and S5 proteins constitute a sub-assembly pathway in the assembly of 30 S subunits in vivo is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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