The phosphorylcholine acceptor in the phosphatidylcholine: ceramide cholinephosphotransferase reaction is the enzyme a transferase or a hydrolase?
| Autor: | Wolf-Dieter Marggraf, Julian N. Kanfer |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Ceramide
Hot Temperature Biophysics Ceramides Biochemistry Substrate Specificity Diglycerides Mice chemistry.chemical_compound Endocrinology Hydrolase Animals Manganese Sphingosine Phospholipase C Phosphorylcholine Phosphotransferases Serum Albumin Bovine Lipid signaling Hydrogen-Ion Concentration Ceramide cholinephosphotransferase Sphingomyelins Kinetics chemistry Type C Phospholipases Diacylglycerol Cholinephosphotransferase lipids (amino acids peptides and proteins) Sphingomyelin |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 793:346-353 |
| ISSN: | 0005-2760 |
| Popis: | The cholinephosphotransferase reaction is shown to be catalyzed by an enzyme which has no hydrolytic activity and which is different from a phospholipase C type activity also present in these plasma membrane preparations. Diacylglycerols and sphingosine, at a concentration above 0.4 mM, are effective inhibitors of sphingomyelin formation in the presence of 0.3 mM free ceramide, the true acceptor in this reaction. Free sphingosine is not an acceptor for the cholinephosphate group, as the anticipated reaction product, sphingosylphosphocholine, could not be detected. Sphingosine inhibition may result from its structural similarity to the natural substrates of the reaction, ceramide and diacylglycerols. From the data obtained with cholesterol, triacylglycerols, acetylated (triacetyl) sphingosine and acetylated ceramides used as potential inhibitors of the reaction it is concluded that the free hydroxyl group at C1 of the sphingosine backbone or of the glycerol moiety of diacylglycerols and a non-polar residue consisting of an aliphatic chain were prerequisites for inhibitory activity. These results are discussed in terms of substrate specificity of the enzyme catalyzing the transfer reaction. Some of the factors influencing the regulation of the phosphatidylcholine/sphingomyelin ratio in the plasma membrane were related to the topography of sphingomyelin in the outer half-layer of the plasma membrane. |
| Databáze: | OpenAIRE |
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