Direct Transverse Relaxation Time Biosensing Strategy for Detecting Foodborne Pathogens through Enzyme-Mediated Sol–Gel Transition of Hydrogels
| Autor: | Luyu Wei, Caiwei Feng, Yunlei Xianyu, Zhilong Wang, Yiping Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Pathogen detection Alginates 010401 analytical chemistry Hydrogels Biosensing Techniques Alkaline Phosphatase 010402 general chemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry Diffusion Enzyme chemistry Transverse Relaxation Time Self-healing hydrogels Biophysics Alkaline phosphatase Molecule Biosensor Sol-gel |
| Zdroj: | Analytical Chemistry. 93:6613-6619 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.0c03968 |
| Popis: | In this work, we develop a direct transverse relaxation time (T2) biosensing strategy and employ it for assaying foodborne pathogens relying on the alkaline phosphatase (ALP)-mediated sol-gel transition of hydrogels. ALP can catalyze the reaction to generate an acidic environment to transform the sol-state alginate solution to hydrogel, and this hydrogelation process can directly regulate the diffusion rate of water protons that results in a T2 change of water molecules. By means of enzyme-modulated sol-gel transition and antigen-antibody interactions, this T2 biosensor displays high sensitivity for detecting 50 CFU/mL S. enteritidis within 2 h. This biosensing strategy directly modulates the water molecules rather than magnetic probes in traditional methods, offering a straightforward, novel, and sensitive platform for pathogen detection. |
| Databáze: | OpenAIRE |
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