Effect of retinoic acid on human adrenal corticosteroid synthesis

Autor: Maria Francesca Cassarino, Francesca Pecori Giraldi, Antonella Sesta, Luigi Castelli, Laura Tapella, Francesco Cavagnini
Rok vydání: 2016
Předmět:
0301 basic medicine
Cortisol secretion
endocrine system
medicine.medical_specialty
Hydrocortisone
Adenoma
medicine.drug_class
Primary Cell Culture
Retinoic acid
Cushing's syndrome
Gene Expression
MC2R
Tretinoin
Adrenocorticotropic hormone
Biology
Cortisol
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
Pharmacology
Toxicology and Pharmaceutics(all)
Adrenocorticotropic Hormone
Internal medicine
Adrenal Glands
medicine
Humans
ACTH receptor
Cholesterol Side-Chain Cleavage Enzyme
Adrenal
General Pharmacology
Toxicology and Pharmaceutics
STAR
Dose-Response Relationship
Drug
Biochemistry
Genetics and Molecular Biology(all)
General Medicine
Phosphoproteins
medicine.disease
030104 developmental biology
Endocrinology
chemistry
Corticosteroid
Receptor
Melanocortin
Type 1
medicine.drug
Zdroj: Life Sciences. 151:277-280
ISSN: 0024-3205
DOI: 10.1016/j.lfs.2016.03.023
Popis: AimsRetinoic acid has recently yielded promising results in the treatment of Cushing's disease, i.e., excess cortisol secretion due to a pituitary corticotropin (ACTH)-secreting adenoma. In addition to its effect on the tumoral corticotrope cell, clinical results suggest an additional adrenal site of action. Aim of this study was to evaluate whether retinoic acid modulates cortisol synthesis and secretion by human adrenals in vitro.Main methodsPrimary cultures from 10 human adrenals specimens were incubated with 10nM, 100nM and 1μM retinoic acid with and without 10nM ACTH for 24h. Cortisol levels were measured by radioimmunoassay and CYP11A1, STAR and MC2R gene expression analyzed by real-time PCR.Key findingsRetinoic acid increased cortisol secretion (149.5±33.01%, 151.3±49.45% and 129.3±8.32% control secretion for 10nM, 100nM and 1μM respectively, p
Databáze: OpenAIRE
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