In Vitro Development of Mouse Embryonic Stem Cells Lacking JNK/Stress-activated Protein Kinase-associated Protein 1 (JSAP1) Scaffold Protein Revealed Its Requirement during Early Embryonic Neurogenesis
| Autor: | Ping Xu, Tomoko Nishioka, Yusaku Nakabeppu, Yohei Tominaga, Katsuji Yoshioka, Michihiko Ito, Daisuke Yoshimura |
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| Rok vydání: | 2003 |
| Předmět: |
Time Factors
animal structures Cell Survival Immunoblotting Retinoic acid Mice Transgenic Nerve Tissue Proteins Tretinoin Ectoderm Embryoid body Biology Biochemistry Cell Line Mice chemistry.chemical_compound Mitogen-Activated Protein Kinase 10 In Situ Nick-End Labeling medicine Animals RNA Messenger WNT1 Molecular Biology Adaptor Proteins Signal Transducing Neurons Recombination Genetic Microscopy Confocal Models Genetic Neuroectoderm Reverse Transcriptase Polymerase Chain Reaction Stem Cells Neurogenesis Wild type Cell Differentiation Cell Biology Protein-Tyrosine Kinases Embryo Mammalian Immunohistochemistry Precipitin Tests Embryonic stem cell Molecular biology Protein Structure Tertiary medicine.anatomical_structure chemistry embryonic structures Mitogen-Activated Protein Kinases Carrier Proteins |
| Zdroj: | Journal of Biological Chemistry. 278:48422-48433 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m307888200 |
| Popis: | The Jsap1 gene encodes a scaffold protein for c-Jun N-terminal kinase cascades. We established c-Jun N-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1 (JSAP1)-null mouse embryonic stem cell lines by homologous recombination. The JSAP1-null embryonic stem cells were viable, however, exhibited hyperplasia of the ectoderm during embryoid body formation, and spontaneously differentiated into neurons more efficiently than did wild type. The expression of components of c-Jun N-terminal kinase cascades and a subset of marker mRNAs during early embryogenesis was altered in the JSAP1-null mutants. Retinoic acid dramatically increased the expression of JSAP1 and JNK3, which were co-precipitated with anti-JNK3 in the neuroectoderm of wild type but not JSAP1-null embryoid bodies. In the neurons differentiated from the wild type embryoid bodies, JSAP1 was localized in the soma, neurites, and growth cone-like structure of the neurites, and neurite outgrowth from the JSAP1-null embryoid bodies was apparently less efficient than from wild type. JSAP1 and c-Jun N-terminal kinase 3 were coexpressed in the embryonic ectoderm of E7.5 mouse embryo, whereas Wnt1 and Pax2 were coexpressed with JSAP1 at the midbrain-hindbrain junction in E12.5 mouse embryo, thus suggesting that JSAP1 is required for early embryonic neurogenesis. |
| Databáze: | OpenAIRE |
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