Selective induction of enhanced complement receptor 3 activity on murine macrophages
| Autor: | William S. Walker, Shing-Erh Yen |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
musculoskeletal diseases Exudate Hot Temperature Physiology Clinical Biochemistry Macrophage-1 Antigen Bone Marrow Cells chemical and pharmacologic phenomena Complement receptor Biology Mice Peritoneal cavity parasitic diseases medicine Animals Ascitic Fluid Trypsin Progenitor cell Receptor Mice Inbred C3H Macrophages hemic and immune systems Cell Biology Molecular biology Receptors Complement Phenotype medicine.anatomical_structure Immunology iC3b Female Bone marrow medicine.symptom medicine.drug |
| Zdroj: | Journal of Cellular Physiology. 115:61-66 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.1041150110 |
| Popis: | A high proportion of murine resident peritoneal macrophages bear complement receptors 1 and 3 (CR1, CR3) which bind C3b and iC3b components of complement, respectively. By contrast, macrophages derived from bone marrow, blood, and the elicited peritoneal exudate are predominantly CR1+3. To determine if the microenvironment of the normal peritoneal cavity influences CR3 phenotype, we studied the effects of lavage from the cavity on cultures of primary peritoneal exudate macrophages, and on macrophages derived from progenitors in the bone marrow, blood, and peritoneal exudate. The cell-free peritoneal lavage (CFPL), after 24 hr of culture, induced CR3 on primary and culture-derived populations of peritoneal exudate macrophages but had no effect on the CR3 phenotype of macrophages derived from bone marrow or blood. The CR3-inducing activity in CFPL was abolished by heating at 70 degrees C for 30 min and by trypsin, and was not affected by adsorption with EA(IgM)iC3b indicator cells, demonstrating that it is not soluble CR3. Finally, exudate macrophages exposed to CFPL required at least 24 hr before they expressed CR3; such macrophages regenerated CR3 after the receptors were removed by trypsin. The selective effect of the activity in CFPL for peritoneal exudate macrophages indicates that the local microenvironment of the peritoneal cavity can influence the expression of CR3. |
| Databáze: | OpenAIRE |
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