Genome-Wide CRISPRi-Based Identification of Targets for Decoupling Growth from Production

Autor:	Nikolaus Sonnenschein, Sheila Ingemann Jensen, Lasse Ebdrup Pedersen, Jenny Marie Landberg, Songyuan Li, Alex Toftgaard Nielsen, Christian Bille Jendresen
Rok vydání:	2020
Předmět:	0106 biological sciences education Biomedical Engineering Biomass 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Genome 03 medical and health sciences 010608 biotechnology Escherichia coli Production (economics) Product formation Clustered Regularly Interspaced Short Palindromic Repeats 030304 developmental biology Carbon flux Gene Library Gene Editing 0303 health sciences Chemistry Escherichia coli Proteins food and beverages General Medicine Gene Expression Regulation Bacterial Bioproduction Metabolic Engineering Biochemical engineering Decoupling (electronics) Genome Bacterial RNA Guide Kinetoplastida
Zdroj:	ACS synthetic biology. 9(5)
ISSN:	2161-5063
Popis:	Growth decoupling can be used to optimize microbial production of biobased compounds by inhibiting excess biomass formation and redirect carbon flux from growth to product formation. However, identifying suitable genetic targets through rational design is challenging. Here, we conduct a genome-wide CRISPRi screen to discover growth switches suitable for decoupling growth and production. Using an sgRNA library covering 12 238 loci in the
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8673777b8623a23cfe29a56cf0406fac https://pubmed.ncbi.nlm.nih.gov/32268068 Zobrazit plný text záznamu