Iron-58 and Neutron Activation Analysis: A Non-Radioactive Method for Tracing Hemoglobin Iron
| Autor: | Greenburg Ag, Y J Yu, Hae Won Kim |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Isotope Stable isotope ratio Chemistry Iron Radiochemistry Biomedical Engineering Neutron Activation Analysis Iron Isotopes Neutron temperature Rats Rats Sprague-Dawley Hemoglobins Blood Substitutes Evaluation Studies as Topic Animals Neutron Hemoglobin Neutron activation analysis Quantitative analysis (chemistry) Biotechnology Gamma counter |
| Zdroj: | Artificial Cells, Blood Substitutes, and Biotechnology. 22:619-624 |
| ISSN: | 1532-4184 1073-1199 |
| DOI: | 10.3109/10731199409117891 |
| Popis: | To develop a non-radioisotopic alternative to radioactive Fe-59 as a hemoglobin iron tracer, we evaluated Fe-58 and neutron activation analysis (NAA). Upon irradiation with thermal neutrons, stable isotope Fe-58 is converted to radioactive Fe-59 through (n,gamma) neutron reaction; its radioactivity measured by a gamma counter is proportional to the original Fe-58 present. Organ samples from rats infused with Hb or saline were analyzed along with a 10 mg Hb as a standard (105 nanograms of Fe-58). The measured values of Fe-58 in tissues were compared with those derived from the literature tissue iron contents. The amount of Fe-58 in normal tissue is extremely low yet detectable by NAA demonstrating high sensitivity. Measured values of Fe-58 from tissues were generally correlated well with literature derived values (e.g., brain: 0.19 and 0.15 ng/mg tissue; kidney: 0.6 and 0.7 ng/mg tissue, respectively). The results indicate that Fe-58 can be used as a non-radioactive tracer for absorption, distribution, metabolism, and distribution studies of hemoglobin-based oxygen carriers. |
| Databáze: | OpenAIRE |
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