Rat hepatic CYP1A1 and CYP1A2 induction by menadione
| Autor: | A.Y. Grishanova, V.V. Lyakhovich, Yulia Sidorova |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Aryl hydrocarbon receptor nuclear translocator Administration Oral Toxicology Isozyme chemistry.chemical_compound Menadione Cytochrome P-450 CYP1A2 In vivo Internal medicine Cytochrome P-450 CYP1A1 medicine Animals RNA Messenger Rats Wistar biology Reverse Transcriptase Polymerase Chain Reaction Aryl Hydrocarbon Receptor Nuclear Translocator CYP1A2 Vitamin K 3 Cytochrome P450 General Medicine MRNA stabilization respiratory system Molecular biology Actins Rats DNA-Binding Proteins Endocrinology Receptors Aryl Hydrocarbon chemistry Enzyme Induction Microsomes Liver Microsome biology.protein Transcription Factors |
| Zdroj: | Toxicology Letters. 155:253-258 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/j.toxlet.2004.10.001 |
| Popis: | The effects of menadione on activities and expression of cytochrome P450 (CYP) 1A subfamily (CYP1A) isozymes in rat hepatic tissue were examined. When rats were treated orally with 15 mg/kg menadione for 4 days, the elevation of hepatic CYP1A1/1A2 specific activities in microsomal preparations was detected with approximately 5.4- and 2.5-fold increase over control values for ethoxyresorufin-O-deethylase (EROD, CYP1A1) and methoxyresorufin-O-demethylase (MROD, CYP1A2) activities, respectively. CYP1A1 and CYP1A2 mRNA levels in the liver of menadione-treated rats were approximately 11.8- and 1.8-fold higher than in controls, respectively, whereas the expression of the CYP1A regulatory proteins aryl hydrocarbon-receptor (AhR) and AhR nuclear translocator (Arnt) was not changed at the mRNA level. The result of this study demonstrates that menadione induces CYP1A1/1A2 expression in vivo through either transcriptional activation and/or mRNA stabilization. |
| Databáze: | OpenAIRE |
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