Association of initiation factor eIF-2 with a rapidly sedimenting fraction from Ehrlich ascites-tumour cells
| Autor: | V M Pain, M J Clemens |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Eukaryotic Initiation Factor-2
Fraction (chemistry) RNA Transfer Amino Acyl Biology Ehrlich ascites Biochemistry RNA Transfer Peptide Initiation Factors Centrifugation Density Gradient medicine Animals Initiation factor Neoplasm Carcinoma Ehrlich Tumor Molecular Biology Cells Cultured Proteins Cell Biology Metabolism medicine.disease Subcellular distribution Microsome Guanosine Triphosphate Protein Binding Subcellular Fractions Research Article |
| Zdroj: | Biochemical Journal. 194:357-360 |
| ISSN: | 0264-6021 |
| DOI: | 10.1042/bj1940357 |
| Popis: | To determine the subcellular distribution of initiation-factor eIF-2 activity, Ehrlich ascites-cell homogenates were fractionated to give (a) a rapidly sedimenting fraction, (b) a microsomal fraction and (c) post-microsomal supernatant. The first two fractions were washed in 0.5 m-KCl to render the associated protein-synthesis factor soluble. As much as 60% of the total recoverable eIF-2 was obtained from the rapidly sedimenting material. |
| Databáze: | OpenAIRE |
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