Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine Quantum Dots
| Autor: | Takashi Kawakami, Alice Y. Ting, Christine K. Payne, Amy E. Jablonski |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Chemistry
technology industry and agriculture Nanotechnology equipment and supplies Article law.invention Membrane Quantum dot Confocal microscopy law Live cell imaging Fluorescence microscope Extracellular Biophysics General Materials Science Physical and Theoretical Chemistry Intracellular Biophysical chemistry |
| Zdroj: | The Journal of Physical Chemistry Letters. 1:1312-1315 |
| ISSN: | 1948-7185 |
| DOI: | 10.1021/jz100248c |
| Popis: | The intracellular, cytosolic, delivery of quantum dots is an important goal for cellular imaging. Recently, a hydrophobic anion, pyrenebutyrate had been proposed to serve as a delivery agent for cationic quantum dots as characterized by confocal microscopy. Using an extracellular quantum dot quencher, QSY-21, as an alternative to confocal microscopy, we demonstrate that quantum dots remain on the cell surface and do not cross the plasma membrane following pyrenebutyrate treatment, a result that is confirmed with transmission electron microscopy. Pyrenebutyrate leads to increased cellular binding of quantum dots rather than intracellular delivery. These results characterize the use of QSY-21 as a quantum dot quencher and highlight the importance of the use of complementary techniques when using confocal microscopy. |
| Databáze: | OpenAIRE |
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