Effects of macrocyclic trichothecene congeners on the viability and mitogenesis of murine splenic lymphocytes
| Autor: | Bruce B. Jarvis, Raghubir P. Sharma, Brian J. Hughes |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
Cellular immunity Cell Survival Lymphocyte Trichothecene Spleen Biology Toxicology Lymphocyte Activation Mice medicine Animals Viability assay Lymphocytes Cytotoxicity Molecular Structure Pokeweed mitogen General Medicine Molecular biology medicine.anatomical_structure Biochemistry Concanavalin A biology.protein Trichothecenes Sesquiterpenes |
| Zdroj: | Toxicology letters. 50(1) |
| ISSN: | 0378-4274 |
| Popis: | The effects of several congeners of the macrocyclic class of trichothecene mycptoxins on murine splenic cells in vitro were investigated. The mycotoxins were roritoxin B, myrotoxin B, roridins A, D and E, baccharinoids B4, B5 and B12, 16-hydroxyverrucarin A, and verrucarins A and J. Lymphocytes from CD-1 mice were cultured with each of the mycotoxins for 48 h to assess cytotoxicity. The maximum effect of various trichothecenes produced on cells occurred at concentrations ranging from 10 −6 to 10 −4 M. Mycotoxins had no effect at concentrations ranging from 10 −12 to 10 −7 M. The mitogenic stimulants concanavalin A, lipopolysaccharide, phytohemagglutinin, and pokeweed mitogen were added to splenic lymphocyte cultures along with varying concentrations of selected mycotoxins. Blastogenesis was inhibited at concentrations 2–5 orders of magnitude lower than those which produced lethality on resting lymphocytes. |
| Databáze: | OpenAIRE |
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