zapERtrap: A light-regulated ER release system reveals unexpected neuronal trafficking pathways
| Autor: | Aaron B. Bowen, Christina S. Winborn, Dean J Kareemo, Thomas Schwarz, Amos Gutnick, Mason S Kleinjan, Samantha L Schwartz, Ashley M. Bourke, Matthew J. Kennedy |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Cell signaling Light Cell Adhesion Molecules Neuronal Primary Cell Culture Gene Expression Golgi Apparatus Biology Endoplasmic Reticulum Hippocampus Synaptic Transmission Cell membrane Rats Sprague-Dawley Tacrolimus Binding Proteins 03 medical and health sciences 0302 clinical medicine Organelle medicine Animals Receptors AMPA Integral membrane protein Secretory pathway 030304 developmental biology Fluorescent Dyes Neurons 0303 health sciences Secretory Pathway Endoplasmic reticulum Cell Membrane Cell Biology Axon initial segment Transport protein Cell biology Molecular Imaging Rats Protein Transport Tetrahydrofolate Dehydrogenase medicine.anatomical_structure Animals Newborn Synapses Female 030217 neurology & neurosurgery |
| Zdroj: | The Journal of cell biology. 220(9) |
| ISSN: | 1540-8140 |
| Popis: | Here we introduce zapalog-mediated endoplasmic reticulum trap (zapERtrap), which allows one to use light to precisely trigger forward trafficking of diverse integral membrane proteins from internal secretory organelles to the cell surface with single cell and subcellular spatial resolution. To demonstrate its utility, we use zapERtrap in neurons to dissect where synaptic proteins emerge at the cell surface when processed through central (cell body) or remote (dendrites) secretory pathways. We reveal rapid and direct long-range trafficking of centrally processed proteins deep into the dendritic arbor to synaptic sites. Select proteins were also trafficked to the plasma membrane of the axon initial segment, revealing a novel surface trafficking hotspot. Proteins locally processed through dendritic secretory networks were widely dispersed before surface insertion, challenging assumptions for precise trafficking at remote sites. These experiments provide new insights into compartmentalized secretory trafficking and showcase the tunability and spatiotemporal control of zapERtrap, which will have broad applications for regulating cell signaling and function. |
| Databáze: | OpenAIRE |
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