Simple and sensitive method for determination of glycoalkaloids in potato tubers by high-performance liquid chromatography with chemiluminescence detection
| Autor: | Shigeo Yamazaki, Youichi Tanaka, Nobumitsu Niina, Keiitsu Saito, Hitoshi Kodamatani |
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| Rok vydání: | 2005 |
| Předmět: |
Detection limit
Chromatography Luminescence Solanine Chemistry Calibration curve Organic Chemistry General Medicine Biochemistry High-performance liquid chromatography Sensitivity and Specificity Analytical Chemistry law.invention chemistry.chemical_compound Alkaloids law Calibration Chaconine Derivatization Quantitative analysis (chemistry) Chromatography High Pressure Liquid Chemiluminescence Solanum tuberosum |
| Zdroj: | Journal of chromatography. A. 1100(1) |
| ISSN: | 0021-9673 |
| Popis: | A novel, simple and sensitive high-performance liquid chromatographic method for the determination of the potato glycoalkaloids, alpha-solanine and alpha-chaconine, based on the chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) has been developed. The calibration graph was linear in the range of 5 ng/ml-10 microg/ml for both alpha-solanine and alpha-chaconine. The detection limits of alpha-solanine and alpha-chaconine were 1.2 and 1.3 ng/ml, respectively. This method was successfully applied to a potato tuber sample without cleanup, pre-concentration, and derivatization steps. The recoveries (mean +/- standard deviation, %) of alpha-solanine and alpha-chaconine spiked in tuber pith at 10 microg/g (n = 6) were 101.0 +/- 4.4% and 103.6 +/- 7.1%, respectively. |
| Databáze: | OpenAIRE |
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