Intracellular and plasma cytokine profile in neonates born to non-atopic parents: the impact of breast feeding
| Autor: | Panagiotis Mavridis, Anna Taparkou, V Drossou-Agakidou, Vasiliki Tzimouli, Chariklia Noutsia, G Kremenopoulos, Florence Kanakoudi-Tsakalidou |
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| Rok vydání: | 2004 |
| Předmět: |
Interleukin 2
Allergy medicine.medical_specialty T-Lymphocytes Enzyme-Linked Immunosorbent Assay Gastroenterology Atopy Interferon-gamma Internal medicine Immunopathology Blood plasma medicine Humans Interferon gamma business.industry Infant Newborn Interleukin Infant medicine.disease Flow Cytometry Endocrinology Breast Feeding Pediatrics Perinatology and Child Health Cytokines Interleukin-2 Interleukin-4 business Breast feeding medicine.drug |
| Zdroj: | European journal of pediatrics. 163(7) |
| ISSN: | 0340-6199 |
| Popis: | The aim of this study was to profile the changes in intracellular and plasma cytokines during the neonatal period and evaluate the impact of breast feeding on these parameters. For this purpose, we measured the interleukin (IL)-2 and IL-4 producing CD3+/CD69+ T-cells using flow cytometry and plasma concentrations of interferon (IFN)-gamma and IL-4 using ELISA, in 122 healthy term neonates, aged 6–12 h, born to non-atopic parents, and 25 healthy children aged 1–12 years. A total of 42/122 neonates exclusively breast-fed (BF) and 39/122 formula fed (FF) were studied again on the 30th day of life for the above parameters. Finally, a clinical evaluation for the presence of atopic disease was conducted at 2 years of age. We found that at birth, the percentage of CD3+/CD69+/IL-4+ T-cells (median = 15.8%, range = 4.4%–49%) and plasma concentrations of IL-4 (median = 0.22 pg/ml, range = 0.18–0.25 pg/ml) were significantly higher (P |
| Databáze: | OpenAIRE |
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