Comparison of chemical constituents of Bupleurum marginatum var. stenophyllum and Bupleurum chinense DC. using UHPLC-Q-TOF-MS based on a metabonomics approach
| Autor: | Xue-Mei Qin, Han Wang, Pan He, Feng Mali, Xi Xiaohu, Liu Xia, Zhaodi Xia, Liguo Tong |
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| Rok vydání: | 2021 |
| Předmět: |
Uhplc q tof ms
Clinical Biochemistry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Drug Discovery Partial least squares regression Metabolomics Molecular Biology Chromatography High Pressure Liquid Pharmacology Principal Component Analysis Chromatography biology Chemistry 010401 analytical chemistry General Medicine Linear discriminant analysis biology.organism_classification Bupleuri radix 0104 chemical sciences Bupleurum Chemical constituents Bupleurum chinense Principal component analysis Bupleurum marginatum Metabolome Drugs Chinese Herbal |
| Zdroj: | Biomedical chromatography : BMCREFERENCES. 35(9) |
| ISSN: | 1099-0801 |
| Popis: | The overall chemical composition of Bupleurum marginatum var. stenophyllum and Bupleurum chinense DC. was compared in this study. Metabolites were identified using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Multivariate statistical analysis techniques such as principal component analysis were used to conduct metabonomics analysis and study the correlation between different components. Principal component analysis results showed a clear distinction among medicinal materials of different origins and divided them into different categories, consistent with the results of hierarchical cluster analysis. Both partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) showed that the two materials could be distinguished clearly. Using PLS-DA and OPLS-DA combined with the S-plot and a variable importance in the projection (VIP) score >1, 24 differential metabolites were screened and identified; all of the metabolites were triterpenoid saponins. In addition, SPSS 25.0 and Metabo Analyst 4.0 were used to analyze significant differences in the relative contents of different compounds in the two materials. This study has successfully provided not only a new direction for research based on the chemical substances identified and the quality evaluation of Bupleuri Radix but also a better theoretical basis for the expansion of medicinal sources and their clinical application. |
| Databáze: | OpenAIRE |
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