Holographic projection for multi-spot structured illumination microscopy
| Autor: | Edward N. Ward, Robert Pal |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Imagination
Histology Computer science media_common.quotation_subject Holography ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION 02 engineering and technology Pathology and Forensic Medicine law.invention 03 medical and health sciences Optics law Microscopy Projection (set theory) 030304 developmental biology media_common ComputingMethodologies_COMPUTERGRAPHICS 0303 health sciences business.industry Resolution (electron density) Potential method 021001 nanoscience & nanotechnology 0210 nano-technology business Phase modulation Excitation |
| Zdroj: | Journal of microscopy, 2019, Vol.274(2), pp.114-120 [Peer Reviewed Journal] |
| Popis: | This paper describes the design and testing of a multispot structured illumination microscopy system using computer-generated holograms to create the required excitation patterns. Furthermore, it demonstrates the use of an adapted direct search algorithm for calculating the holograms that allows for imaging across an extended field of view. The system was tested on fixed targets and live cells yielding a two times resolution increase over conventional diffraction-limited imaging. LAY DESCRIPTION: We present the design and testing of a multispot structured illumination microscopy system using computer-generated holograms to create the required excitation patterns. It demonstrates the use of an adapted direct search algorithm for calculating the holograms that allows for imaging across an extended field of view. The system was tested on fixed targets and live cells yielding a two times resolution increase over conventional diffraction-limited imaging. The results here demonstrate that holography provides an efficient means of pattern projection for MSIM imaging. It provides a significant improvement in the efficiency of pattern projection and more importantly it allows for the testing of more diverse excitation patterns than possible with amplitude-only projection. For example, PSF engineering using phase modulation can be easily incorporated into the calculated holograms, potentially generating subdiffraction structures in the excitation pattern. The ability to incorporate PSF engineering into SIM opens up holographic MSIM as a potential method for further increasing resolution with little or no change to the imaging system. |
| Databáze: | OpenAIRE |
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