Identification of a Regulated Alkaline Phosphatase, a Cell Surface-Associated Lipoprotein, in Mycobacterium smegmatis
| Autor: | Sunhee Lee, Jordan Kriakov, William R. Jacobs |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Signal peptide
Transposable element Sequence analysis Operon Immunoprecipitation Lipoproteins Phosphatase Molecular Sequence Data Mycobacterium smegmatis Genetics and Molecular Biology Microbiology Phosphates Bacterial Proteins Molecular Biology biology Cell Membrane Gene Expression Regulation Bacterial Sequence Analysis DNA biology.organism_classification Alkaline Phosphatase Molecular biology Mutagenesis Insertional Biochemistry DNA Transposable Elements Alkaline phosphatase bacteria |
| Popis: | Although alkaline phosphatases are common in a wide variety of bacteria, there has been no prior evidence for alkaline phosphatases in Mycobacterium smegmatis . Here we report that transposon insertions in the pst operon, encoding homologues of an inorganic phosphate transporter, leads to constitutive expression of a protein with alkaline phosphatase activity. DNA sequence analysis revealed that M. smegmatis does indeed have a phoA gene that shows high homology to other phoA genes. The M. smegmatis phoA gene was shown to be induced by phosphate starvation and thus negatively regulated by the pst operon. Interestingly, the putative M. smegmatis PhoA has a hydrophobic N-terminal domain which resembles a lipoprotein signal sequence. The M. smegmatis PhoA was demonstrated to be an exported protein associated with the cell surface. Furthermore, immunoprecipitation of PhoA from [ 14 C]acetate-labeled M. smegmatis cell lysates demonstrated that this phosphatase is a lipoprotein. |
| Databáze: | OpenAIRE |
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