Construction of SARS-CoV-2 spike-pseudotyped retroviral vector inducing syncytia formation

Autor: Se Yeong Lee, Do Woo Kim, Yong Tae Jung
Rok vydání: 2022
Předmět:
Zdroj: Virus Genes. 58:172-179
ISSN: 1572-994X
0920-8569
DOI: 10.1007/s11262-022-01890-z
Popis: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is handled in biosafety level 3 (BSL-3) facilities, whereas the antiviral screening of pseudotype virus is conducted in BSL-2 facilities. In this study, we developed a SARS-CoV-2 spike-pseudotyped virus based on a semi-replication-competent retroviral (s-RCR) vector system. The s-RCR vector system was divided into two packageable vectors, each with gag-pol and env genes. For env vector construction, SARS-CoV-2 SΔ19 env was inserted into the pCLXSN-IRES-EGFP retroviral vector to generate pCLXSN-SΔ19 env-EGFP. When pCLXSN-gag-pol and pCLXSN-SΔ19env-EGFP were co-transfected into HEK293 T cells to generate an s-RCR virus, titers of the s-RCR virus were consistently low in this transient transfection system (1 × 10
Databáze: OpenAIRE
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