BMP2 induces hMSC osteogenesis and matrix remodeling
| Autor: | Wei Wang, Ji Zou, Aofei Yang, Hantao Cai |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Bone Morphogenetic Protein 2 Biochemistry Bone morphogenetic protein 2 Chondrocyte Cell Line 03 medical and health sciences 0302 clinical medicine Osteogenesis Bone cell Genetics medicine Humans Molecular Biology N-cadherin Bone morphogenesis mesenchymal stem cells Chemistry Mesenchymal stem cell Cell Differentiation Osteoblast Articles osteoporosis Extracellular Matrix Cell biology isobaric tags for relative and absolute quantification 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Oncology 030220 oncology & carcinogenesis Molecular Medicine Bone marrow Extracellular matrix organization |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2020.11764 |
| Popis: | With increasing age, the microenvironment in the bone marrow is altered, leading to a decrease in bone marrow mesenchymal stem cell (BMSC) differentiation, which reduces the number of bone cells and weakens osteogenic capacity, resulting in osteoporosis (OP). The clinical manifestations of OP include bone loss, bone microstructural destruction and altered bone quality. Bone morphogenetic protein 2 (BMP2) serves an important role in inducing the osteogenic differentiation of mesenchymal stem cells (MSCs). Regulating the bone marrow matrix microenvironment and promoting osteogenic differentiation of BMSCs is of significance for both the prevention and treatment of OP. In the present study, isobaric tags for relative and absolute quantification (iTRAQ) high-throughput proteomics technology was combined with bioinformatics analysis to screen 249 differentially expressed proteins in human MSCs overexpressing BMP2, of which 173 were upregulated and 76 proteins were downregulated. The proteins were also involved in signaling pathways associated with extracellular matrix organization, osteoblast differentiation, ossification, bone development, chondrocyte differentiation and bone morphogenesis. By carefully screening the proteins, N-cadherin (CDH2), a protein with osteogenic differentiation potential, was verified by perturbations in the background of BMP2 overexpression. The role of CDH3 in the osteogenic differentiation of MSCs was confirmed by the regulation of several cognate osteogenic markers, suggesting CDH2 as a promising candidate in the field of osteogenesis. |
| Databáze: | OpenAIRE |
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