MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma
| Autor: | Ren-Tao Zhao, Yang Chen, Li Chen, Fan-bin Meng, Xiao Li, Wei Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Sp1 Transcription Factor
Blotting Western Cell Down-Regulation Biology Cell Line Cell Movement Cell Line Tumor microRNA Genetics medicine Humans 3' Untranslated Regions Molecular Biology Genetics (clinical) Cell Proliferation Regulation of gene expression Osteosarcoma Reverse Transcriptase Polymerase Chain Reaction Cell growth Cell Cycle Transfection Cell cycle Gene Expression Regulation Neoplastic MicroRNAs medicine.anatomical_structure Cell culture Apoptosis Cancer research |
| Zdroj: | Cytogenetic and Genome Research. 146:124-135 |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000437379 |
| Popis: | miR-150 expression in osteosarcoma (OS) cell lines and human osteoblast cells was detected, and OS cell models were transfected with exogenous miR-150 to investigate its role in cell proliferation, invasion, and apoptosis. Our results showed that miR-150 expression in OS cells (MG63, Saos-2, SOSP-9607, and U2OS) was significantly lower compared to the osteoblast hFOB1.19 cell line (all p < 0.01). The expression level of miR-150 in MG63 cells that were transfected with exogenous miR-150 mimics was markedly upregulated, while the miR-150 expression level in the inhibitor group was significantly downregulated (both p < 0.01). Similar results were also found in SOSP-9607 cells. Importantly, exogenous miR-150 expression stimulated cell apoptosis and inhibited proliferation, invasion, and migration. A luciferase reporter assay displayed that miR-150 also regulated Sp1 expression by targeting its 3′-UTR, and qRT-PCR and Western blotting showed that elevated levels of miR-150 may reduce Sp1 protein expression. The mRNA and protein levels of Sp1 were upregulated after transfection with a Sp1-expression plasmid and partially reversed the inhibitory effects of miR-150 on cell proliferation, invasion, and metastasis in MG63 and SOSP-9607 cells, as well as promoted cell apoptosis. In conclusion, miR-150 inhibits cell proliferation, invasion, and metastasis and stimulates cell apoptosis by regulating the expression of Sp1. Therefore, miR-150 may be a potential clinical target for the treatment of OS patients. |
| Databáze: | OpenAIRE |
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