Pumping supplies alter the microbiome of pumped human milk: An in-home, randomized, crossover trial
| Autor: | Michelle K. McGuire, Anthony G. Hay, Janet E. Williams, Mark A. McGuire, Sarah M. Reyes, Kathleen M. Rasmussen, Dainelle L Allen |
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| Rok vydání: | 2021 |
| Předmět: |
Cross-Over Studies
Nutrition and Dietetics Bacteria Milk Human Aerobic bacteria Microbiota Breastfeeding Infant Medicine (miscellaneous) Infant health Biology Acinetobacter biology.organism_classification Crossover study Bacterial counts Original Research Communications Breast Feeding Animal science RNA Ribosomal 16S Humans Female Microbiome Collection methods |
| Zdroj: | Am J Clin Nutr |
| ISSN: | 0002-9165 |
| DOI: | 10.1093/ajcn/nqab273 |
| Popis: | BACKGROUND: The human milk microbiome may contribute to the benefits of breastfeeding by providing bacteria to the infant gastrointestinal tract. Many women pump their milk, but the effect of pumping on the milk microbiome is unknown. OBJECTIVES: Our objective was to determine the effects of pumping supplies on the pumped human milk microbiome. METHODS: This was an in-home, randomized, crossover trial of 2 collection methods. Women (n = 52) pumped twice within 3.5 h, once with their own breast pumps and milk collection supplies (OWN SUPP) and once with a hospital-grade pump and sterile collection supplies (STER SUPP). Pumping order was randomized. The milk microbiome was characterized by aerobic culturing and 16S ribosomal RNA gene sequencing. RESULTS: Milk collected with OWN SUPP yielded more total aerobic and gram-negative bacteria than milk collected with STER SUPP, reflecting a 6.6 (adjusted OR; 95% CI: 1.7, 25; P = 0.006) higher odds of containing >10(4) total aerobic CFU/mL and 19 (adjusted OR; 95% CI: 4.1, 88; P |
| Databáze: | OpenAIRE |
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