Growth regulation and co-stimulation of human colorectal cancer cell lines by insulin-like growth factor I, II and transforming growth factor alpha
| Autor: | A. Ceyhan, N. Odartchenko, J.-C. Givel, P. L. Laurent, L. Suardet, Harald Lahm, J. R. Fischer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Cancer Research
medicine.medical_specialty TGF alpha medicine.medical_treatment Biology In Vitro Techniques Cell Line Insulin-like growth factor Insulin-Like Growth Factor II Internal medicine medicine Humans Insulin-Like Growth Factor I Serum Albumin Dose-Response Relationship Drug Cell growth Growth factor Drug Synergism Transforming Growth Factor alpha Molecular biology Cytokine Endocrinology Oncology Cell culture Insulin-like growth factor 2 biology.protein Colorectal Neoplasms Cell Division Transforming growth factor Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | We have tested growth factor responsiveness of a panel of eight human colorectal carcinoma cell lines. Insulin-like growth factors I and II (IGF-I and IGF-II) stimulated growth of five lines (HT-29, LS411N, LS513, SW480, WiDr). At 30 ng ml-1 both factors enhanced growth up to 3-fold. They induced half-maximal stimulation at 1.9-6.51 ng ml-1. Even after delayed addition IGF-I and II significantly enhanced growth in a short-term proliferation assay. They exerted maximal effects under limiting serum conditions (0.5% FCS) and at low cell density (1.25-5 x 10(4) ml-1). Using these conditions transforming growth factor alpha (TGF alpha) enhanced proliferation of all IGF-responsive cell lines, except SW480. 1.11-3.31 ng ml-1 were required to obtain a half-maximal response. With 10-20 ng ml-1 maximal stimulation occurred at plateau values different from those for IGF-I/II. Proliferation of all cell lines responsive to both IGF-I and TGF alpha was further enhanced by combining both factors, resulting a synergistic response of LS513, while the effects on HT-29, LS411N and WiDr were additive. With HT-29 and LS411N a 24 h exposure to TGF alpha was sufficient to obtain a full response in the co-stimulatory assay. Our results illustrate the importance of IGF-I/II and TGF alpha as stimulators of growth of colorectal carcinomas. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|