A quantity control mechanism regulating levels of the HapE subunit of the Hap complex in Aspergillus nidulans: no accumulation of HapE in hapC deletion mutants
| Autor: | R. Oonishi, Norihiro Tsukagoshi, S. Tanoue, Fumiko Naruse, Masashi Kato, K. Hayashi, Tetsuo Kobayashi, Akimitsu Tanaka, Y. Tateyama |
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| Rok vydání: | 2002 |
| Předmět: |
Protein subunit
Biophysics Hap complex medicine.disease_cause Subunit assembly Biochemistry Aspergillus nidulans law.invention Fungal Proteins Western blot stomatognathic system Structural Biology law Gene Expression Regulation Fungal Genetics medicine Northern blot RNA Messenger Molecular Biology Gene Mutation biology medicine.diagnostic_test CCAAT-binding complex RNA Cell Biology biology.organism_classification Molecular biology Protein Subunits CCAAT-Binding Factor Recombinant DNA Dimerization Gene Deletion |
| Zdroj: | FEBS letters. 512(1-3) |
| ISSN: | 0014-5793 |
| Popis: | The Aspergillus nidulans CCAAT-binding complex (Hap complex) consists of at least three subunits, HapB, HapC and HapE. To investigate the quantity control mechanisms of the subunits during assembly of the Hap complex, reconstitution studies with the recombinant subunits and extracts prepared from the respective hap subunit deletion mutants were carried out. Furthermore, Western blot analysis of the Hap subunits and Northern blot analysis of the hap genes with the respective deletion mutants were also performed. From all the results together, it was suggested that the number of the HapC molecule could adjust that of the HapE molecule by forming stable heterodimers prior to assembly of the Hap complex. |
| Databáze: | OpenAIRE |
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