Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs
| Autor: | Agnès Jamin, Gaëlle Kuntz-Simon, Roland Cariolet, Stéphane Gorin, Marie-Frédérique Le Potier |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
Swine Palatine Tonsil antigen presenting cells 0403 veterinary science Interferon [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Cells Cultured 0303 health sciences Immunity Cellular [SDV.BA]Life Sciences [q-bio]/Animal biology 04 agricultural and veterinary sciences interferon E2 antigen 3. Good health Specific Pathogen-Free Organisms medicine.anatomical_structure [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Classical Swine Fever Virus Organ Specificity secondary lymphoid organs Cytokines [SDV.IMM]Life Sciences [q-bio]/Immunology Tumor necrosis factor alpha Female [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] medicine.drug Gene Expression Regulation Viral 040301 veterinary sciences T cell Spleen [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Classical Swine Fever 03 medical and health sciences Immune system Antigen Antigens CD medicine Animals Viremia 030304 developmental biology pestivirus General Veterinary Interleukins Germinal center [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Dendritic cell Dendritic Cells [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics Immunology Antibody Formation [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie |
| Zdroj: | Veterinary Research Veterinary Research, BioMed Central, 2008, 39 (1), pp.1-17. ⟨10.1051/vetres:2007045⟩ |
| ISSN: | 0928-4249 1297-9716 |
| DOI: | 10.1051/vetres:2007045⟩ |
| Popis: | International audience; Classical swine fever virus (CSFV) compromises the host immune system, causing indirect leucopoenia and disruption of in vitro T cell stimulation capacity. In order to explore the potential role of dendritic cells (DC) in such phenomena, the activation of conventional DC (cDC) and plasmacytoid DC (pDC) in blood and secondary lymphoid organs of infected pigs was investigated in the early time course post-inoculation (pi), together with viral components dissemination and cytokine production in serum. Whereas CD11R1$^{+}$CD172a$^{+}$ cDC frequencies were markedly reduced in blood and spleen, analysis of CD4$^{+}$CD172a$^{+}$ pDC numbers revealed a rapid turn-over of this DC subset in tissues pi. Both subsets matured and were activated after infection, as demonstrated by down-regulation of CD1a, up-regulation of the co-stimulation molecule CD80/86 and expression of cytokines. cDC essentially expressed tumor necrosis factor alpha (TNF-$\alpha$) and interleukin (IL)-10, whereas pDC produced alpha interferon (IFN-$\alpha$) and IL-12. IFN-$\alpha $ and TNF-$\alpha $ productions revealed an enhancement of innate anti-viral immune responses. Detection of antigen activated B lymphocytes in tonsil T-cell areas at 72 h pi, subsequently to the transient translocation of the viral E2 protein within germinal centres at 48 h pi, indicates the initiation of humoral response. This response was also evidenced by an important IL-10 production in serum one week pi. IL-12 expression in organs, as well as transient detection of IL-18 and IFN-$\gamma $ in serum, reflected the initiation of cellular immune responses. However, the uncommonly high levels of TNF-$\alpha $ and IFN-$\alpha $ produced by DC and measured in serum early post-infection, together with IL-10 expression in spleen, could play a role in the disruption of immune system cells, either inducing apoptosis or impairing DC functionalities themselves. |
| Databáze: | OpenAIRE |
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