Posttranslational Regulation of IL-23 Production Distinguishes the Innate Immune Responses to Live Toxigenic versus Heat-Inactivated Vibrio cholerae
Autor: | Daniel L. Bourque, Ana A. Weil, Jason B. Harris, Stephen B. Calderwood, Taufiqur Rahman Bhuiyan, Ashraful Islam Khan, Fahima Chowdhury, Meti D. Debela, Edward T. Ryan, Richelle C. Charles, Crystal N. Ellis, Regina C. LaRocque, Firdausi Qadri, Rasheduzzaman Rashu |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Molecular Biology and Physiology Cholera Toxin Vaccines Live Unattenuated Hot Temperature THP-1 Cells 030231 tropical medicine cholera Biology medicine.disease_cause Microbiology Monocytes 03 medical and health sciences 0302 clinical medicine Immune system IL-23 Immunity medicine Humans RNA Processing Post-Transcriptional Vibrio cholerae Molecular Biology Antigens Bacterial Innate immune system Toxin Cholera toxin Cholera Vaccines medicine.disease Antibodies Bacterial Cholera Immunity Innate QR1-502 3. Good health 030104 developmental biology Gene Expression Regulation Vaccines Inactivated Interleukin-23 Subunit p19 Cytokines Cholera vaccine Research Article |
Zdroj: | mSphere, Vol 4, Iss 4 (2019) mSphere |
ISSN: | 2379-5042 |
Popis: | An episode of cholera provides better protection against reinfection than oral cholera vaccines, and the reasons for this are still under study. To better understand this, we compared the immune responses of human cells exposed to live Vibrio cholerae with those of cells exposed to heat-killed V. cholerae (similar to the contents of oral cholera vaccines). We also compared the effects of active cholera toxin and the inactive cholera toxin B subunit (which is included in some cholera vaccines). One key immune signaling molecule, IL-23, was uniquely produced in response to the combination of live bacteria and active cholera holotoxin. Stimulation with V. cholerae that did not produce the active toxin or was killed did not produce an IL-23 response. The stimulation of IL-23 production by cholera toxin-producing V. cholerae may be important in conferring long-term immunity after cholera. Vibrio cholerae infection provides long-lasting protective immunity, while oral, inactivated cholera vaccines (OCV) result in more-limited protection. To identify characteristics of the innate immune response that may distinguish natural V. cholerae infection from OCV, we stimulated differentiated, macrophage-like THP-1 cells with live versus heat-inactivated V. cholerae with and without endogenous or exogenous cholera holotoxin (CT). Interleukin 23A gene (IL23A) expression was higher in cells exposed to live V. cholerae than in cells exposed to inactivated organisms (mean change, 38-fold; 95% confidence interval [95% CI], 4.0 to 42; P |
Databáze: | OpenAIRE |
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