Tumor suppressive microRNA-200a inhibits renal cell carcinoma development by directly targeting TGFB2
| Autor: | Ziliang Ji, Chunjuan Zhao, Qingna Zhai, Guanghui Cui, Xiaoqing Li, Wei Zhang, Jing Chen, Zhendong Yu, Ruijing Lu, Jie Qin |
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| Rok vydání: | 2015 |
| Předmět: |
Epithelial-Mesenchymal Transition
Tumor initiation Biology law.invention Gene Knockout Techniques Transforming Growth Factor beta2 Cell Movement Renal cell carcinoma law Cell Line Tumor microRNA medicine Humans Neoplasm Invasiveness RNA Small Interfering Carcinoma Renal Cell Cell Proliferation Gene knockdown Cell growth General Medicine medicine.disease Molecular biology Gene Expression Regulation Neoplastic MicroRNAs Cell culture Cancer research Suppressor Transforming growth factor |
| Zdroj: | Tumor Biology. 36:6691-6700 |
| ISSN: | 1423-0380 1010-4283 |
| DOI: | 10.1007/s13277-015-3355-9 |
| Popis: | A large body of evidence indicates that microRNAs play a critical role in tumor initiation and progression by negatively regulating oncogenes or tumor suppressor genes. Here, we report that the expression of miR-200a was notably downregulated in 45 renal cell carcinoma (RCC) samples. Restoration of miR-200a suppressed cell proliferation, migration, and invasion in two RCC cell lines. Furthermore, we used an epithelial-to-mesenchymal transition PCR array to explore the putative target genes of miR-200a. By performing quantitative real-time PCR, ELISA, and luciferase reporter assays, transforming growth factor beta2 (TGFB2) was validated as a direct target gene of miR-200a. Moreover, siRNA-mediated knockdown of TGFB2 partially phenocopied the effect of miR-200a overexpression. These results suggest that miR-200a suppresses RCC development via directly targeting TGFB2, indicating that miR-200a may present a novel target for diagnostic and therapeutic strategies in RCC. |
| Databáze: | OpenAIRE |
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