Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents

Autor: Brenda Alvarado Sánchez, Manuel Jiménez-Estrada, Verónica Jiménez-Suárez, Antonio Nieto-Camacho
Rok vydání: 2016
Předmět:
0301 basic medicine
Hamelia
Time Factors
Antioxidant
DPPH
medicine.medical_treatment
Anti-Inflammatory Agents
Pharmaceutical Science
Pharmacology
Carrageenan
chemistry.chemical_compound
0302 clinical medicine
Hamelia patens
Drug Discovery
Edema
Stigmasterol
Traditional medicine
biology
Free Radical Scavengers
General Medicine
030220 oncology & carcinogenesis
Tetradecanoylphorbol Acetate
Molecular Medicine
Female
Saccharomyces cerevisiae Proteins
medicine.drug_class
Context (language use)
Anti-inflammatory
03 medical and health sciences
Picrates
medicine
Animals
Glycoside Hydrolase Inhibitors
Rats
Wistar
Peroxidase
Plants
Medicinal
Dose-Response Relationship
Drug
business.industry
Biphenyl Compounds
alpha-Glucosidases
biology.organism_classification
Plant Leaves
Disease Models
Animal
030104 developmental biology
Complementary and alternative medicine
chemistry
Alpha-glucosidase
Solvents
biology.protein
business
Phytotherapy
Zdroj: Pharmaceutical Biology. 54:1822-1830
ISSN: 1744-5116
1388-0209
DOI: 10.3109/13880209.2015.1129544
Popis: Context Hamelia patens Jacq. (Rubiaceae) is traditionally used to treat wounds, inflammation and diabetes. However, there is still a lack of scientific evidence to support these applications. Objective The objective of this study is to evaluate the anti-inflammatory, antioxidant and antidiabetic activities of Hamelia patens, and identify its bioactive compounds. Materials and methods Four extracts were obtained by maceration and liquid-liquid extraction: HEX, DCM-EtOAc, MeOH-EtOAc and MeOH-Aq. The anti-inflammatory effect was evaluated orally on rat paw carrageenan-induced oedema over 6 h (50, 200 and 500 mg/kg), and topically in mouse ear oedema induced by 12-tetradecanoylphorbol-13-acetate (TPA) after 4 h (0.5 and 1 mg/ear). We also evaluated myeloperoxidase levels in ear tissue, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability, and in vitro α-glucosidase inhibition. The chemical compounds were separated by column chromatography and identified by spectroscopic analysis. Results We found that the oral administration of the HEX extract at 500 and 200 mg/kg significantly decreased the carrageenan-induced inflammation after 1 and 3 h, respectively. The MeOH-EtOAc extract significantly inhibited myeloperoxidase activity (83.5%), followed by the DCM-EtOAc extract (76%), β-sitosterol/stigmasterol (72.7%) and the HEX extract (55%), which significantly decreased oedema induced by TPA at both doses, giving a similar effect to indomethacin. We also found that the MeOH-EtOAc, MeOH-Aq and DCM-EtOAc extracts showed good DPPH scavenging activity (IC50 values of 18.6, 93.9 and 158.2 μg/mL, respectively). The HEX extract showed the lowest α-glucosidase inhibition (an IC50 value of 26.07 μg/mL), followed by the MeOH-EtOAc extract (an IC50 value of 30.18 μg/mL), β-sitosterol/stigmasterol (IC50 34.6 μg/mL) and compound A ((6E,10E,14E,18E)-2,6,10,14,18,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene, an IC50 value of 114.6 μg/mL), which were isolated for the first time from Hamelia patens. Discussion and conclusion Hamelia patens possesses anti-inflammatory, antioxidant and α-glucosidase inhibitory activities, which support its traditional use. These effects can be attributed to the identified compounds.
Databáze: OpenAIRE
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